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Light and Video Microscopy

Academic Press Title
ISBN: 978-0-12-374234-6
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Light and Video Microscopy

By Randy O. Wayne

312 pages
Trim Size 8 1/2 X 10 7/8 in
Copyright 2009
USD 104.00, Hardcover

Available: In Stock

 
Key Features

  • Brings together mathematics, physics, and biology to provide a broad and deep understanding of the light microscope

  • Clearly develops all ideas from historical and logical foundations

  • Laboratory exercises included to assist the reader with practical applications

  • Microscope discussions include: bright field microscope, dark field microscope, oblique illumination, phase-contrast microscope, photomicrography, fluorescence microscope, polarization microscope, interference microscope, differential interference microscope, and modulation contrast microscope

    Related Titles

    Description

    Most information about the nature of living cells is discovered through light microscope observation. The image seen through a microscope is not a magnified point-by-point representation of the object, but is an abstract representation of the object that must be interpreted. This interpretation is influenced not only by the actual structure of the object, but the physical characteristics of light and the microscope components, the preparation of the sample, and the microscopist's background. In order to interpret the image correctly and fully, the microscopist must understand the interaction of light with the sample.

    The purpose of this book is to provide the most comprehensive, easy-to-use, and informative guide on light microscopy. Light and Video Microscopy will prepare the reader for accurate interpretation of an image and understanding of the living cell. With the presentation of geometrical optics, it will assist the reader in understanding image formation and light movement within the microscope. It also provides an explanation of the basic modes of light microscopy and the components of modern electronic imaging systems and guides the reader in determining the physicochemical information of living and developing cells which influence interpretation.


    Readership

    cell biologists, neurobiologists, reproductive biologists, plant biologists, microbiologists, fungal biologists, eterinarians, forensic scientists, food scientists, horticulturalists, biochemists, physicists, engineers, and anyone using a light or video microscope

    Contents

    (tentative)


    Chapter 1: The Relation Between the Object and the Image

    Chapter 2: Geometrical Optics

    Chapter 3: Physical Optics

    Chapter 4: Bright Field Microscopy

    Chapter 5: Phase Contrast and Dark Field Microscopy, Rheinberg and Oblique Illumination

    Chapter 6: Photomicrography

    Chapter 7: Fluorescence Microscopy

    Chapter 8: Polarization Microscopy

    Chapter 9: Interference Microscopy

    Chapter 10: Differential Interference Microscopy

    Chapter 11: Modulation Contrast Microscopy

    Chapter 12: Video and Digital Microscopy

    Chapter 13: Image Processing and Analysis

    Chapter 14: Various Kinds of Microscopes and Accessories

    Author Information

    By Randy O. Wayne, Department of Plant Biology
    Cornell University, Ithaca, NY, USA

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